Open AccessFlavobacterium johnsoniae SprA Is a Cell Surface Protein Involved in Gliding Motility
Author(s) -
Shawn S. Nelson,
Padden P. Glocka,
Sakshi Agarwal,
David P. Grimm,
Mark J. McBride
Publication year - 2007
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00892-07
Subject(s) - biology , biophysics , motility , cell , mutant , microbiology and biotechnology , biochemistry , gene
Flavobacterium johnsoniae cells glide rapidly over surfaces by an unknown mechanism. Transposon-inducedsprA mutants formed nonspreading colonies on agar, and the cells examined in wet mounts were deficient in attachment to surfaces and were almost completely nonmotile. Exposure of intact cells to proteinase K cleaved the 270-kDa SprA into several large peptides, suggesting that it is partially exposed on the cell surface.