Open AccessPrimer on Agar-Based Microbial Imaging Mass Spectrometry
Author(s) -
Jane Y. Yang,
Vanessa V. Phelan,
Ryan Simkovsky,
Jeramie D. Watrous,
Rachelle M. Trial,
Tinya C. Fleming,
Roland Wenter,
Bradley S. Moore,
Susan S. Golden,
Kit Pogliano,
Pieter C. Dorrestein
Publication year - 2012
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00823-12
Subject(s) - biology , mass spectrometry , mass spectrometry imaging , agar , sample preparation , computational biology , matrix assisted laser desorption/ionization , chromatography , bacteria , genetics , desorption , chemistry , organic chemistry , adsorption
Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) imaging mass spectrometry (IMS) applied directly to microbes on agar-based medium captures global information about microbial molecules, allowing for direct correlation of chemotypes to phenotypes. This tool was developed to investigate metabolic exchange factors of intraspecies, interspecies, and polymicrobial interactions. Based on our experience of the thousands of images we have generated in the laboratory, we present five steps of microbial IMS: culturing, matrix application, dehydration of the sample, data acquisition, and data analysis/interpretation. We also address the common challenges encountered during sample preparation, matrix selection and application, and sample adherence to the MALDI target plate. With the practical guidelines described herein, microbial IMS use can be extended to bio-based agricultural, biofuel, diagnostic, and therapeutic discovery applications.
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