Open AccessFliT Acts as an Anti-FlhD 2 C 2 Factor in the Transcriptional Control of the Flagellar Regulon in Salmonella enterica Serovar Typhimurium
Author(s) -
Shouji Yamamoto,
Kazuhiro Kutsukake
Publication year - 2006
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00799-06
Subject(s) - regulon , biology , microbiology and biotechnology , promoter , salmonella enterica , operon , gene , transcription factor , genetics , gene expression , escherichia coli
Flagellar operons are divided into three classes with respect to their transcriptional hierarchy inSalmonella enterica serovar Typhimurium. The class 1 gene products FlhD and FlhC act together in an FlhD2 C2 heterotetramer, which binds upstream of the class 2 promoters to facilitate binding of RNA polymerase. Class 2 expression is known to be enhanced by a disruption mutation in a flagellar gene,fliT . In this study, we purified FliT protein in a His-tagged form and showed that the protein prevented binding of FlhD2 C2 to the class 2 promoter and inhibited FlhD2 C2 -dependent transcription. Pull-down and far-Western blotting analyses revealed that the FliT protein was capable of binding to FlhD2 C2 and FlhC and not to FlhD alone. We conclude that FliT acts as an anti-FlhD2 C2 factor, which binds to FlhD2 C2 through interaction with the FlhC subunit and inhibits its binding to the class 2 promoter.