Genetic Analysis of Mutacin B-Ny266, a Lantibiotic Active against Caries Pathogens

Bacteriocins are ribosomally synthesized proteinaceous antibacterial peptides. They selectively interfere with the growth of other bacteria. The production and secretion of bacteriocins confer a distinct ecological advantage to the producer in competing against other bacteria that are present in the same ecological niche. , a significant contributor to the development of dental caries, is one of the most prolific producer of bacteriocins, known as mutacins in In this study, we characterized the locus encoding mutacin B-Ny266, a lantibiotic with a broad-spectrum of activity. The chromosomal locus is composed of six predicted operon structures encoding proteins involved in regulation, antimicrobial activity, biosynthesis, modification, transport, and immunity. Mutacin B-Ny266 was purified from semi-solid cultures and two inhibitory peptides, LanA and LanA', were detected. Both peptides were highly modified. Such modifications include dehydration of serine and threonine, and the formation of a C-terminal aminovinyl-cysteine (AviCys) ring. While LanA peptide alone is absolutely required for antimicrobial activity, the presence of LanA' enhanced the activity of LanA, suggesting that B-Ny266 may function as a two-peptide lantibiotic. The activation of expression is most likely controlled by the conserved two-component system NsrRS that is activated by LanA peptide but not LanA'. The chromosomal locus encoding mutacin B-Ny266 was not universally conserved in all sequenced genomes. Intriguingly, the genes encoding LanAA' peptides were restricted to the most invasive serotypes of Although dental caries is largely preventable, it remains the most common and costly infectious diseases worldwide. Caries is initiated by the presence of dental plaque biofilm that contains , a species extensively characterized for its role in caries development and formation. deploys an arsenal of strategies to establish itself within the oral cavity. One of them is the production of bacteriocins that confer a competitive advantage by targeting and killing closely related competitors. In this work, we found that mutacin B-Ny266 is a potent lantibiotic effective at killing a wide-array of oral streptococci, including nearly all strains tested. Lantibiotics produced by oral bacteria could represent a promising strategy to target caries pathogens embedded in dental plaque biofilm.