Open AccessResidues Involved in FecR Binding Are Localized on One Side of the FecA Signaling Domain in Escherichia coli
Author(s) -
Elena B. M. Breidenstein,
Susanne Mahren,
Volkmar Braun
Publication year - 2006
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00741-06
Subject(s) - biology , escherichia coli , escherichia coli proteins , signaling proteins , plasma protein binding , domain (mathematical analysis) , genetics , signal transduction , biochemistry , gene , mathematical analysis , mathematics
Ferric citrate transport inEscherichia coli involves proteins encoded by thefec genes, including the transport and signaling protein FecA and the signal transducing protein FecR. Randomly isolated FecA point mutants showed a reduced interaction with FecR and a reduced transcription initiation of the ferric citrate transport genes. The mutations were localized on one side of the FecA signaling domain, which might form the interface to FecR. Some of the mutants showed strongly reduced iron transport rates, which suggests that the signaling domain affects the structure of the FecA transporter domain.