Open AccessA Genome-Wide Helicobacter pylori Morphology Screen Uncovers a Membrane-Spanning Helical Cell Shape Complex
Author(s) -
Desirée C. Yang,
Kris M. Blair,
Jennifer A. Taylor,
Timothy W. Petersen,
Tate H. Sessler,
Christina M. Tull,
Christina K. Leverich,
Amanda L. Collar,
Timna J.O. Wyckoff,
Jacob Biboy,
Waldemar Vollmer,
Nina R. Salama
Publication year - 2019
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00724-18
Subject(s) - biology , pentapeptide repeat , mutant , peptidoglycan , helicobacter pylori , microbiology and biotechnology , cell , cell membrane , gene , biochemistry , genetics , peptide
The stomach ulcer and cancer-causing pathogenHelicobacter pylori has a helical cell shape which facilitates stomach infection. Using light scattering to measure perturbations of cell morphology, we identified 28 genes that influence different aspects of cell shape. A mutant in a previously uncharacterized protein renamed Csd7 failed to form any helical cells. Biochemical analyses showed that Csd7 collaborates with other proteins to stabilize the cell wall-degrading enzyme Csd1. Csd7 also links Csd1 with a putative filament-forming protein via protein-protein interactions. These data suggest that helical cell shape arises from a highly orchestrated program to regulate cell wall modifications. Targeting of this helical cell shape-promoting program could offer new ways to block infectivity of this important human pathogen.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom