SigF Controls Carotenoid Pigment Production and Affects Transformation Efficiency and Hydrogen Peroxide Sensitivity in Mycobacterium smegmatis | Zendy

Roberta Provvedi | Zendy; Dana Kocíncová | Zendy; Valentina Donà | Zendy; Daniel Euphrasie | Zendy; Mamadou Daffé | Zendy; Gilles Etienne | Zendy; Riccardo Manganelli | Zendy; JeanMarc Reyrat | Zendy

Open Access

SigF Controls Carotenoid Pigment Production and Affects Transformation Efficiency and Hydrogen Peroxide Sensitivity in Mycobacterium smegmatis

Author(s) -

Roberta Provvedi,

Dana Kocíncová,

Valentina Donà,

Daniel Euphrasie,

Mamadou Daffé,

Gilles Etienne,

Riccardo Manganelli,

JeanMarc Reyrat

Publication year - 2008

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.00714-08

Subject(s) - mycobacterium smegmatis , biology , carotenoid , mutant , hydrogen peroxide , pigment , biochemistry , mycobacterium , microbiology and biotechnology , bacteria , genetics , mycobacterium tuberculosis , gene , chemistry , medicine , tuberculosis , organic chemistry , pathology

Carotenoids are complex lipids that are known for acting against photodynamic injury and free radicals. We demonstrate here that sigma(F) is required for carotenoid pigment production in Mycobacterium smegmatis. We further show that a sigF mutant exhibits a transformation efficiency 10(4)-fold higher than that of the parental strain, suggesting that sigma(F) regulates the production of components affecting cell wall permeability. In addition, a sigF mutant showed an increased sensitivity to hydrogen peroxide. An in silico search of the M. smegmatis genome identified a number of SigF consensus sites, including sites upstream of the carotenoid synthesis locus, which explains its SigF regulation.

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