Open AccessInfluence of the Two-Component System SaeRS on Global Gene Expression in Two Different Staphylococcus aureus Strains
Author(s) -
Kathrin Rogasch,
Vanessa Rühmling,
Jan PanéFarré,
Dirk W. Höper,
Christin Weinberg,
Stephan Fuchs,
Mareike Schmudde,
Barbara M. Bröker,
Christiane Wolz,
Michael Hecker,
Susanne Engelmann
Publication year - 2006
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00555-06
Subject(s) - biology , staphylococcus aureus , microbiology and biotechnology , gene , gene expression , staphylococcus , component (thermodynamics) , bacterial protein , expression (computer science) , bacteria , staphylococcal infections , genetics , physics , computer science , programming language , thermodynamics
The two-component system SaeRS consisting of the histidin kinase SaeS and the response regulator SaeR is known to act on virulence gene expression in Staphylococcus aureus. In order to get a more comprehensive picture on SaeR-regulated genes, we studied the contribution of the two-component system on global gene expression by using both the proteomic and transcriptomic approach. Altogether, a loss of SaeRS resulted in a decreased amount of at least 17 extracellular proteins and two cell surface-associated proteins, among them several important virulence factors such as HlgA, HlgB, HlgC, LukF, and LukM. SaeRS activates the expression of these genes at the transcriptional level. The amount of the five proteins Aur, SspA, SsaA, Plc, and GlpQ was negatively influenced by SaeRS. However, the transcription of the corresponding genes was not affected by the two-component system. SaeRS had also no measurable influence on the transcription of the regulatory genes agr, sarA, arlRS, and sigB that contribute to the regulation of SaeRS-dependent virulence factors identified in this investigation. Our results clearly show that SaeRS is strongly involved in the tight temporal control of virulence factor expression in S. aureus. Its precise role within the regulatory network remains to be determined.