Tripartite Regulation of the glpFKD Operon Involved in Glycerol Catabolism by GylR, Crp, and SigF in Mycobacterium smegmatis | Zendy

Hyun-Ju Bong | Zendy; Eon-Min Ko | Zendy; Su-Yeon Song | Zendy; In-Jeong Ko | Zendy; JeongIl Oh | Zendy

Open Access

Tripartite Regulation of the glpFKD Operon Involved in Glycerol Catabolism by GylR, Crp, and SigF in Mycobacterium smegmatis

Author(s) -

Hyun-Ju Bong,

Eon-Min Ko,

Su-Yeon Song,

In-Jeong Ko,

JeongIl Oh

Publication year - 2019

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.00511-19

Subject(s) - mycobacterium smegmatis , biology , operon , mycobacterium , catabolism , microbiology and biotechnology , glycerol , eubacterium , bacteria , biochemistry , genetics , mycobacterium tuberculosis , metabolism , gene , escherichia coli , tuberculosis , medicine , pathology

Using genetic approaches, we first revealed that glycerol is catabolized through the glycolytic pathway after conversion to dihydroxyacetone phosphate in two sequential reactions catalyzed by glycerol kinase (GlpK) and flavin adenine dinucleotide (FAD)-containing glycerol-3-phosphate dehydrogenase (GlpD) inM. smegmatis . Our study also revealed that in addition to the GylR transcriptional activator that mediates the induction of theglpFKD operon by glycerol, the operon is regulated by SigF and Crp, which reflect the cellular energy state and cAMP level, respectively.

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