Open AccessDesT Coordinates the Expression of Anaerobic and Aerobic Pathways for Unsaturated Fatty Acid Biosynthesis in Pseudomonas aeruginosa
Author(s) -
Chitra Subramanian,
Charles O. Rock,
Yongmei Zhang
Publication year - 2010
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00404-09
Subject(s) - operon , biology , biochemistry , pseudomonas aeruginosa , fatty acid , gene , anaerobic exercise , biosynthesis , pseudomonas , microbiology and biotechnology , bacteria , escherichia coli , genetics , physiology
ThefabA andfabB genes are responsible for anaerobic unsaturated fatty acid formation inPseudomonas aeruginosa . Expression of thefabAB operon was repressed by exogenous unsaturated fatty acids, and DNA sequences upstream of the translational start site were used to affinity purify DesT. The single protein interaction with thefabAB promoter detected in wild-type cell extracts was absent in thedesT deletion strain, as was the repression offabAB expression by unsaturated fatty acids. Thus, DesT senses the overall composition of the acyl-coenzyme A pool to coordinate the expression of the operons for the anaerobic (fabAB ) and aerobic (desCB ) pathways for unsaturated fatty acid synthesis.