Development of Transposon Mutagenesis for Chlamydia muridarum | Zendy

Yibing Wang | Zendy; Scott D. LaBrie | Zendy; Steven Carrell | Zendy; Robert J. Suchland | Zendy; Zoe Dimond | Zendy; Forrest Kwong | Zendy; Daniel D. Rockey | Zendy; P. Scott Hefty | Zendy; Kevin Hybiske | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Development of Transposon Mutagenesis for Chlamydia muridarum

Author(s) -

Yibing Wang,

Scott D. LaBrie,

Steven Carrell,

Robert J. Suchland,

Zoe Dimond,

Forrest Kwong,

Daniel D. Rockey,

P. Scott Hefty,

Kevin Hybiske

Publication year - 2019

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.00366-19

Subject(s) - biology , transposable element , transposon mutagenesis , genetics , mutant , orfs , mutagenesis , chlamydia , forward genetics , sleeping beauty transposon system , gene , open reading frame , peptide sequence

Historical issues with genetic manipulation ofChlamydia have prevented rigorous functional genetic characterization of the ∼1,000 genes in chlamydial genomes. Here, we report the development of a transposon mutagenesis system forC. muridarum , a mouse-adaptedChlamydia species that is widely used forin vivo investigations of chlamydial pathogenesis. This advance builds on the pioneering development of this system forC. trachomatis . We demonstrate the generation of an initial library of 33 mutants containing stable single or double transposon insertions. Using these mutant clones, we characterizedin vitro phenotypes associated with genetic disruptions in glycogen biosynthesis and three polymorphic outer membrane proteins.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research