Open AccessCell Cycle Characteristics of Crenarchaeota : Unity among Diversity
Author(s) -
Magnus Lundgren,
Laurence Malandrin,
Stefan Eriksson,
Harald Huber,
Rolf Bernander
Publication year - 2008
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00330-08
Subject(s) - crenarchaeota , biology , archaea , cell division , genetics , cell cycle , microbiology and biotechnology , cell , bacteria
The hyperthermophilic archaeaAcidianus hospitalis ,Aeropyrum pernix ,Pyrobaculum aerophilum ,Pyrobaculum calidifontis , andSulfolobus tokodaii representing three different orders in the phylumCrenarchaeota were analyzed by flow cytometry and combined phase-contrast and epifluorescence microscopy. The overall organization of the cell cycle was found to be similar in all species, with a short prereplicative period and a dominant postreplicative period that accounted for 64 to 77% of the generation time. Thus, in allCrenarchaeota analyzed to date, cell division and initiation of chromosome replication occur in close succession, and a long time interval separates termination of replication from cell division. InPyrobaculum , chromosome segregation overlapped with or closely followed DNA replication, and further genome separation appeared to occur concomitant with cellular growth. Cell division inP. aerophilum took place without visible constriction.