YshB Promotes Intracellular Replication and Is Required for Salmonella Virulence

virulence requires the initial invasion of host cells followed by the modulation of the intracellular environment for survival and replication. In an effort to characterize the role of small RNAs in pathogenesis, we inadvertently identified a 5 kD protein named YshB that is involved in the intracellular survival of We show here that expression is upregulated upon entry into macrophages. When expression is upregulated before bacterial entry, invasion efficiency is inhibited. Lack of YshB resulted in reduced bacterial survival within the macrophages and led to reduced virulence in a mouse model of infection. gastroenteritis is one of the most common causes of food-borne disease, possibly affecting millions of people globally each year. Here we characterize the role of a novel small protein YshB in mediating intracellular survival. This elucidation adds another layer to the body of knowledge of how this bacterium confers its intracellular survival.