Open AccessThe CheZ Binding Interface of CheA S Is Located in α-Helix E
Author(s) -
C. David O’Connor,
Philip Matsumura,
Andrés Campos
Publication year - 2009
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00294-09
Subject(s) - biology , genetics , helix (gastropod) , microbiology and biotechnology , zoology , gastropoda
Specific CheA-short (CheAS ) residues, L123 and L126, were identified as critical for CheZ binding. In the CheAS ′P1-CheZ nuclear magnetic resonance structure, these residues form an interaction surface on α-helix E in the ′P1 domain. Both L123 and L126 are buried in CheA-long (CheAL ), providing an explanation for why CheAL fails to bind CheZ.