Open AccessStructure-Based Functional Analysis of the Replication Protein of Plasmid R6K: Key Amino Acids at the π/DNA Interface
Author(s) -
Selvi Kunnimalaiyaan,
Sheryl A. Rakowski,
Marcin Filutowicz
Publication year - 2007
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00109-07
Subject(s) - biology , plasmid , dna , amino acid , dna replication , biochemistry
In previous work, we characterized the bases in an iteron of plasmid R6K that are important for the binding of π protein monomers and dimers. Here we investigate the following six amino acids of π, encoded bypir , hypothesized to be important for DNA contact: Ser71, Try74, Gly131, Gly211, Arg225, and Arg254.