Expanding the Clostridioides difficile Genetics Toolbox | Zendy

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Expanding the Clostridioides difficile Genetics Toolbox

Author(s) -

Aimee Shen

Publication year - 2019

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.00089-19

Subject(s) - biology , clostridioides , genetics , transposon mutagenesis , microbial genetics , crispr , transposable element , molecular genetics , mutagenesis , gene silencing , computational biology , gene , mutation , mutant , microbiology and biotechnology

Clostridioides difficile genetics has rapidly advanced in recent years thanks to the development of tools for allelic replacement and transposon mutagenesis. In this Journal of Bacteriology issue, Müh et al extend the genetics toolbox by developing a CRISPRi strategy for gene silencing in C. difficile (U.Müh, A. G. Pannullo, D. S. Weiss, and C. D. Ellermeier, 2019, J Bacteriol 201:e00711-18. . https://doi.org/10.1128/JB.00711-18). The authors demonstrate the tunability and robustness of their CRISPRi system, highlight its utility in studying essential gene function, and discuss exciting new possibilities for dissecting C. difficile physiology.

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