Open AccessGenome Sequence of Hybrid V ibrio cholerae O1 MJ-1236, B-33, and CIRS101 and Comparative Genomics with V. cholerae
Author(s) -
Christopher J. Grim,
Nur A. Hasan,
Elisa Taviani,
Bradd J. Haley,
Jongsik Chun,
Thomas Brettin,
David Bruce,
John C. Detter,
Cliff Han,
Olga Chertkov,
Jean F. Challacombe,
Anwar Huq,
G. Balakrish Nair,
Rita R. Colwell
Publication year - 2010
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00040-10
Subject(s) - biology , vibrio cholerae , el tor , prophage , genome , genetics , comparative genomics , whole genome sequencing , clade , strain (injury) , virology , genomics , phylogenetic tree , gene , bacteriophage , bacteria , escherichia coli , anatomy
The genomes ofV ibrio cholerae O1 Matlab variant MJ-1236, Mozambique O1 El Tor variant B33, and altered O1 El Tor CIRS101 were sequenced. All three strains were found to belong to the phylocore group 1 clade ofV. cholerae , which includes the 7th-pandemic O1 El Tor and serogroup O139 isolates, despite displaying certain characteristics of the classical biotype. All three strains were found to harbor a hybrid variant of CTXΦ and an integrative conjugative element (ICE), leading to their establishment as successful clinical clones and the displacement of prototypical O1 El Tor. The absence of strain- and group-specific genomic islands, some of which appear to be prophages and phage-like elements, seems to be the most likely factor in the recent establishment of dominance ofV. cholerae CIRS101 over the other two hybrid strains.