Open AccessActivation and Repression at the Escherichia coli ynfEFGHI Operon Promoter
Author(s) -
Meng Xu,
Stephen Busby,
Douglas F. Browning
Publication year - 2009
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00040-09
Subject(s) - operon , biology , psychological repression , escherichia coli , rna polymerase , activator (genetics) , transcription (linguistics) , promoter , microbiology and biotechnology , gal operon , transcription factor , sigma factor , gene , genetics , gene expression , linguistics , philosophy
Induction of theEscherichia coli K-12ynfEFGHI operon in response to anaerobiosis is repressed by nitrate ions. In this study, we show that the global transcription factor FNR is a class II activator at theynfEFGHI promoter and that NarL represses activation by binding to a single target that overlaps the promoter −10 element. Electromobility shift assays show that NarL does not prevent RNA polymerase binding and suggest that repression may involve a quaternary NarL-FNR-RNA polymerase-promoter complex.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom