A Riboswitch Regulates Expression of the Coenzyme B 12 -Independent Methionine Synthase in Mycobacterium tuberculosis : Implications for Differential Methionine Synthase Function in Strains H37Rv and CDC1551 | Zendy

Digby F. Warner | Zendy; Suzana Savvi | Zendy; Valerie Mizrahi | Zendy; Stephanie S. Dawes | Zendy

Open Access

A Riboswitch Regulates Expression of the Coenzyme B ₁₂ -Independent Methionine Synthase in Mycobacterium tuberculosis : Implications for Differential Methionine Synthase Function in Strains H37Rv and CDC1551

Author(s) -

Digby F. Warner,

Suzana Savvi,

Valerie Mizrahi,

Stephanie S. Dawes

Publication year - 2007

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.00040-07

Subject(s) - methionine synthase , biology , riboswitch , methionine , atp synthase , mycobacterium tuberculosis , microbiology and biotechnology , mutant , coenzyme a , biochemistry , gene , gene expression , enzyme , tuberculosis , amino acid , non coding rna , medicine , pathology , reductase

We observed vitamin B12 -mediated growth inhibition ofMycobacterium tuberculosis strain CDC1551. The B12 sensitivity was mapped to a polymorphism inmetH , encoding a coenzyme B12 -dependent methionine synthase. Vitamin B12 -resistant suppressor mutants of CDC1551 containing mutations in a B12 riboswitch upstream of themetE gene, which encodes a B12 -independent methionine synthase, were isolated. Expression analysis confirmed that the B12 riboswitch is a transcriptional regulator ofmetE inM. tuberculosis .

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