Open AccessIdentification of a Novel Mycobacterial 3-Hydroxyacyl-Thioester Dehydratase, HtdZ (Rv0130), by Functional Complementation in Yeast
Author(s) -
Aner Gurvitz,
Jouni Hiltunen,
Alexander J. Kastaniotis
Publication year - 2008
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00016-08
Subject(s) - dehydratase , complementation , biology , biochemistry , saccharomyces cerevisiae , lipoic acid , mutant , yeast , microbiology and biotechnology , enzyme , gene , antioxidant
We report on the identification ofMycobacterium tuberculosis HtdZ (Rv0130), representing a novel 3-hydroxyacyl-thioester dehydratase. HtdZ was picked up by the functional complementation ofSaccharomyces cerevisiae htd2 Δ cells lacking the dehydratase of mitochondrial type II fatty acid synthase. Mutant cells expressing HtdZ contained dehydratase activity, recovered their respiratory ability, and partially restored de novo lipoic acid synthesis.