Open AccessModification of a Mammalian Cell Protein in the Presence of [ 32 P-Adenylate]NAD: Evidence for ADP Ribosylation Activity Associated with Helicobacter pylori
Author(s) -
Carlos W. Nossa,
Steven R. Blanke
Publication year - 2006
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.74.5.3071-3076.2006
Subject(s) - nad+ kinase , biology , adenylate kinase , adp ribosylation , helicobacter pylori , biochemistry , cell culture , microbiology and biotechnology , enzyme , genetics
Culture filtrates fromHelicobacter pylori promote the transfer of the radiolabel from [32 P-adenylate]NAD to one or more heat-labile factors within extracts prepared from several mammalian cell lines, with the predominate radiolabeled species exhibiting an apparent molecular mass of greater than 130 kDa. Our results suggest that severalH. pylori strains release a factor that ADP-ribosylates a mammalian target protein.