Open AccessRequirement of Histidine Kinases HP0165 and HP1364 for Acid Resistance in Helicobacter pylori
Author(s) -
John T. Loh,
Timothy L. Cover
Publication year - 2006
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.74.5.3052-3059.2006
Subject(s) - biology , histidine kinase , helicobacter pylori , mutant , kinase , histidine , two component regulatory system , wild type , transcription (linguistics) , gene , helicobacter , microbiology and biotechnology , biochemistry , genetics , amino acid , linguistics , philosophy
In this study, we investigated a potential requirement of two-component signal transduction systems for acid resistance inHelicobacter pylori . In comparison to a wild-type strain, isogenic strains with null mutations in either HP0165 or HP1364 histidine kinases were impaired in their ability to grow at pH 5.0. The growth of complemented mutant strains was similar to that of the wild-type strain.H. pylori DNA array analyses and transcriptional reporter assays indicated that acid-responsive gene transcription was altered in the HP0165 and HP1364 null mutant strains compared to the parental wild-type strain. These results indicate that intact HP0165 and HP1364 histidine kinases are required for acid resistance inH. pylori .