Open AccessTransforming Growth Factor β1 Production by CD4+CD25+Regulatory T Cells in Peripheral Blood Mononuclear Cells from Healthy Subjects Stimulated withLeishmania guyanensis
Author(s) -
Amina Kariminia,
Eliane Bourreau,
Hervé Pascalis,
Pierre Couppié,
D. SainteMarie,
Fabienne TacchiniCottier,
Pascal Launois
Publication year - 2005
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.73.9.5908-5914.2005
Subject(s) - il 2 receptor , biology , peripheral blood mononuclear cell , microbiology and biotechnology , interleukin 2 , transforming growth factor beta , transforming growth factor , immune system , immunology , in vitro , t cell , endocrinology , biochemistry
Transforming growth factor β (TGF-β) has been shown to be a central immunomodulator used by leishmaniae to escape effective mechanisms of protection in human and murine infections with these parasites. However, all the information is derived from studies of established infection, while little is known about TGF-β production in response toLeishmania stimulation in healthy subjects. In this study, TGF-β1 production was demonstrated in peripheral blood mononuclear cells from healthy subjects never exposed to leishmaniae in response to liveLeishmania guyanensis , and the TGF-β1-producing cells were described as a distinct subpopulation of CD4+ CD25+ regulatory T cells. The suppressive properties of CD4+ CD25+ T cells were demonstrated in vitro by their inhibition of production of interleukin 2 (IL-2) and IL-10 by CD4+ CD25− T cells in the presence of either anti-CD3 orL. guyanensis . Although neutralization of TGF-β1 did not reverse the suppressive activity of CD4+ CD25+ T cells activated by anti-CD3, it reversed the suppressive activity of CD4+ CD25+ T cells activated byL. guyanensis . Altogether our data demonstrated that TGF-β1 is involved in the suppressive activity ofL. guyanensis -stimulated CD4+ CD25+ T cells from healthy controls.