Open AccessCD4+T Cells Play a Dominant Role in Protection against New World Leishmaniasis Induced by Vaccination with the P-4 Amastigote Antigen
Author(s) -
Sudeshna Kar,
Christine N. Metz,
Diane McMahon-Pratt
Publication year - 2005
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.73.6.3823-3827.2005
Subject(s) - biology , amastigote , tumor necrosis factor alpha , cd8 , immunology , lymphotoxin , leishmania , interferon gamma , antigen , cutaneous leishmaniasis , vaccination , t cell , population , virology , cytotoxic t cell , leishmania major , immune system , in vitro , leishmaniasis , parasite hosting , medicine , biochemistry , environmental health , world wide web , computer science
Immunodepletion studies of P-4-vaccinated mice indicate that CD4+ and not CD8+ T cells are critical for protection against Leishmania pifanoi (Leishmania mexicana complex). Although a moderate CD8+ T-cell response is elicited by vaccination, CD4+ T cells are the dominant responding population in vitro and at the cutaneous site of infection. These protective T cells produce gamma interferon (IFN-gamma), macrophage migration inhibitory factor (MIF), and tumor necrosis factor/lymphotoxin (TNF/LT), each of which significantly contributed to intracellular parasite destruction in vitro. These results indicate that a singular CD4+ T-cell response (IFN-gamma, MIF, and/or LT/TNF) can provide protection against New World cutaneous leishmaniasis.