Open AccessBordetella bronchisepticaFlagellin Is a Proinflammatory Determinant for Airway Epithelial Cells
Author(s) -
Yolanda S. López-Boado,
Laura M. Cobb,
Rajendar Deora
Publication year - 2005
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.73.11.7525-7534.2005
Subject(s) - bordetella bronchiseptica , proinflammatory cytokine , flagellin , biology , microbiology and biotechnology , airway , immunology , bordetella , inflammation , bacteria , medicine , bordetella pertussis , genetics , surgery
Motility is an important virulence phenotype for many bacteria, and flagellin, the monomeric component of flagella, is a potent proinflammatory factor. Of the threeBordetella species,Bordetella pertussis andBordetella parapertussis are nonmotile human pathogens, whileBordetella bronchiseptica expresses flagellin and causes disease in animals and immunocompromised human hosts. The BvgAS two-component signal transduction system regulates phenotypic-phase transition (Bvg+ , Bvg− , and Bvgi ) in bordetellae. The Bvg− phase ofB . bronchiseptica is characterized by the expression of flagellin and the repression of adhesins and toxins necessary for the colonization of the respiratory tract.B. bronchiseptica naturally infects a variety of animal hosts and constitutes an excellent model to studyBordetella pathogenesis. Using in vitro coculture models of bacteria and human lung epithelial cells, we studied the effects ofB. bronchiseptica flagellin on host defense responses. Our results show thatB. bronchiseptica flagellin is a potent proinflammatory factor that induces chemokine, cytokine, and host defense gene expression. Furthermore, we investigated receptor specificity in the response toB. bronchiseptica flagellin. Our results show thatB. bronchiseptica flagellin is able to signal effectively through both human and mouse Toll-like receptor 5.