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Complete Genome Sequence and Comparative Genomics ofShigella flexneriSerotype 2a Strain 2457T
Author(s) -
Jun S. Wei,
Marcia B. Goldberg,
Valerie Burland,
Malabi M. Venkatesan,
Wen-Ling Deng,
Guillaume Fournier,
George F. Mayhew,
Guy Plunkett,
Debra J. Rose,
Aaron E. Darling,
Bob Mau,
Nicole T. Perna,
Shelley M. Payne,
Laura J. Runyen-Janecky,
Shiguo Zhou,
David C. Schwartz,
Frederick R. Blattner
Publication year - 2003
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.71.5.2775-2786.2003
Subject(s) - shigella flexneri , biology , pseudogene , genetics , genome , shigella , comparative genomics , prophage , virulence , whole genome sequencing , gene , microbiology and biotechnology , insertion sequence , serotype , strain (injury) , genomic island , escherichia coli , genomics , bacteriophage , transposable element , anatomy
We determined the complete genome sequence of Shigella flexneri serotype 2a strain 2457T (4,599,354 bp). Shigella species cause >1 million deaths per year from dysentery and diarrhea and have a lifestyle that is markedly different from those of closely related bacteria, including Escherichia coli. The genome exhibits the backbone and island mosaic structure of E. coli pathogens, albeit with much less horizontally transferred DNA and lacking 357 genes present in E. coli. The strain is distinctive in its large complement of insertion sequences, with several genomic rearrangements mediated by insertion sequences, 12 cryptic prophages, 372 pseudogenes, and 195 S. flexneri-specific genes. The 2457T genome was also compared with that of a recently sequenced S. flexneri 2a strain, 301. Our data are consistent with Shigella being phylogenetically indistinguishable from E. coli. The S. flexneri-specific regions contain many genes that could encode proteins with roles in virulence. Analysis of these will reveal the genetic basis for aspects of this pathogenic organism's distinctive lifestyle that have yet to be explained.

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