Open AccessIdentification and Functional Analysis of an Immunoreactive DsbA-Like Thio-Disulfide Oxidoreductase of Ehrlichia spp
Author(s) -
Jere W. McBride,
Lucy Ndip,
Vsevolod L. Popov,
David H. Walker
Publication year - 2002
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.70.5.2700-2703.2002
Subject(s) - dsba , ehrlichia chaffeensis , biology , ehrlichia , virology , microbiology and biotechnology , ehrlichia canis , recombinant dna , escherichia coli , periplasmic space , genetics , antibody , serology , gene , tick
Novel homologous DsbA-like disulfide bond formation (Dsb) proteins of Ehrlichia chaffeensis and Ehrlichia canis were identified which restored DsbA activity in complemented Escherichia coli dsbA mutants. Recombinant Ehrlichia Dsb (eDsb) proteins were recognized by sera from E. canis-infected dogs but not from E. chaffeensis-infected patients. The eDsb proteins were observed primarily in the periplasm of E. chaffeensis and E. canis.