Open AccessConstruction of an Actinobacillus pleuropneumoniae Serotype 2 Prototype Live Negative-Marker Vaccine
Author(s) -
Walaiporn Tonpitak,
Nina Baltes,
Isabel Hennig-Pauka,
Gerald-F. Gerlach
Publication year - 2002
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.70.12.7120-7125.2002
Subject(s) - actinobacillus pleuropneumoniae , biology , serotype , homologous recombination , microbiology and biotechnology , virology , serology , strain (injury) , mutant , gene , genetics , antibody , anatomy
Deletions were introduced into the ureC and apxIIA genes of an Actinobacillus pleuropneumoniae serotype 2 strain by homologous recombination and counterselection. The double-mutant contains no foreign DNA, is highly attenuated, protects pigs from homologous challenge upon a single aerosol application, and facilitates the serological discrimination of immunized and infected herds.