Open AccessProteomics Reveals Open Reading Frames in Mycobacterium tuberculosis H37Rv Not Predicted by Genomics
Author(s) -
Peter R. Jungblut,
EvaChristina Müller,
Jens Mattow,
Stefan H. E. Kaufmann
Publication year - 2001
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.69.9.5905-5907.2001
Subject(s) - proteomics , biology , genomics , mycobacterium tuberculosis , structural genomics , comparative genomics , computational biology , functional genomics , genetics , whole genome sequencing , genome , gene , tuberculosis , microbiology and biotechnology , biochemistry , medicine , pathology , protein structure
Genomics revealed the sequence of 3924 genes of the H37Rv strain of Mycobacterium tuberculosis. Proteomics complements genomics in showing which genes are really expressed, and here we show the expression of six genes not predicted by genomics, as proved by two-dimensional electrophoresis and matrix-assisted laser desorption ionization and nano-electrospray mass spectrometry.