Open AccessIn Vitro and In Vivo Assessment ofSalmonella entericaSerovar Typhimurium DT104 Virulence
Author(s) -
C. Allen,
Paula J. Fedorka–Cray,
Andrés VázquezTorres,
M. Mitsu Suyemoto,
Craig Altier,
L. R. Ryder,
Ferric C. Fang,
Stephen J. Libby
Publication year - 2001
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.69.7.4673-4677.2001
Subject(s) - salmonella enterica , virulence , microbiology and biotechnology , serotype , biology , salmonella , enterobacteriaceae , virology , bacteria , escherichia coli , gene , genetics
Multidrug-resistant Salmonella enterica serovar Typhimurium phage type DT104 has become a widespread cause of human and other animal infection worldwide. The severity of clinical illness in S. enterica serovar Typhimurium DT104 outbreaks has led to the suggestion that this strain possesses enhanced virulence. In the present study, in vitro and in vivo virulence-associated phenotypes of several clinical isolates of S. enterica serovar Typhimurium DT104 were examined and compared to S. enterica serovar Typhimurium ATCC 14028s. The ability of these DT104 isolates to survive within murine peritoneal macrophages, invade cultured epithelial cells, resist antimicrobial actions of reactive oxygen and nitrogen compounds, and cause lethal infection in mice were assessed. Our results failed to demonstrate that S. enterica serovar Typhimurium DT104 isolates are more virulent than S. enterica serovar Typhimurium ATCC 14028s.