Open AccessMolecular and Genomic Analysis of Genes Encoding Surface-Anchored Proteins from Clostridium difficile
Author(s) -
T. Karjalainen,
AnneJudith WaligoraDupriet,
Marina Cerquetti,
Patrizia Spigaglia,
Andrea Maggioni,
Pierluigi Mauri,
Paola Mastrantonio
Publication year - 2001
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.69.5.3442-3446.2001
Subject(s) - biology , bacterial adhesin , gene , genetics , virulence , locus (genetics) , open reading frame , protein domain , clostridium difficile , homology (biology) , peptide sequence , antibiotics
The gene slpA, encoding the S-layer precursor protein in the virulent Clostridium difficile strains C253 and 79--685, was identified. The precursor protein carries a C-terminal highly conserved anchoring domain, similar to the one found in the Cwp66 adhesin (previously characterized in strain 79--685), an SLH domain, and a variable N-terminal domain mediating cell adherence. The genes encoding the S-layer precursor proteins and the Cwp66 adhesin are present in a genetic locus carrying 17 open reading frames, 11 of which encode a similar two-domain architecture, likely to include surface-anchored proteins.