Open AccessIdentification of a Novel Gene Cluster Encoding Staphylococcal Exotoxin-Like Proteins: Characterization of the Prototypic Gene and Its Protein Product, SET1
Author(s) -
Rachel Williams,
John M. Ward,
Brian E. Henderson,
Stephen J. Poole,
B.P. O'Hara,
Michael Wilson,
Sean P. Nair
Publication year - 2000
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.68.8.4407-4415.2000
Subject(s) - biology , exotoxin , gene cluster , gene , staphylococcus aureus , microbiology and biotechnology , pseudomonas exotoxin , locus (genetics) , toxic shock syndrome , homology (biology) , sequence analysis , genetics , recombinant dna , toxin , bacteria
We report the discovery of a novel genetic locus withinStaphylococcus aureus that encodes a cluster of at least five exotoxin-like proteins. Designated the staphylococcal exotoxin-like genes 1 to 5 (set1 toset5 ), these open reading frames have between 38 and 53% homology to each other. All five proteins contain consensus sequences that are found in staphylococcal and streptococcal exotoxins and toxic shock syndrome toxin 1 (TSST-1). However, the SETs have only limited overall sequence homology to the enterotoxins and TSST-1 and thus represent a novel family of exotoxin-like proteins. The prototypic gene in this cluster,set1 , has been cloned and expressed. Recombinant SET1 stimulated the production of interleukin-1β, interleukin-6, and tumor necrosis factor alpha by human peripheral blood mononuclear cells. PCR analysis revealed thatset1 was distributed among other strains ofS. aureus but not in the other staphylococcal species examined. Sequence analysis of theset1 genes from different strains revealed at least three allelic variants. The protein products of these allelic variants displayed a 100-fold difference in their cytokine-inducing potency. The distribution of allelic variants of theset genes among strains ofS. aureus may contribute to differences in the pathogenic potential of this bacterium.