Open AccessImmunization with Parasite-Derived Apical Membrane Antigen 1 or Passive Immunization with a Specific Monoclonal Antibody Protects BALB/c Mice against Lethal Plasmodium yoelii yoelii YM Blood-Stage Infection
Author(s) -
David L. Narum,
Solabomi A. Ogun,
Alan W. Thomas,
Anthony A. Holder
Publication year - 2000
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.68.5.2899-2906.2000
Subject(s) - plasmodium yoelii , rhoptry , biology , monoclonal antibody , immunization , antigen , virology , antibody , immunology , parasite hosting , malaria , plasmodium falciparum , parasitemia , apicomplexa , world wide web , computer science
We have purified apical merozoite antigen 1 (AMA-1) from extracts of red blood cells infected with the rodent malaria parasitePlasmodium yoelii yoelii YM. When used to immunize mice, the protein induced a strong protective response against a challenge with the parasite. Monoclonal antibodies specific forP. yoelii yoelii AMA-1 were prepared, and one was very effective against the parasite on passive immunization. A second protein that appears to be located in the apical rhoptry organelles and associated with AMA-1 was identified.