Open AccessStable Expression of a New Chimeric Fluorescent Reporter in the Human Malaria Parasite Plasmodium falciparum
Author(s) -
Madhusudan Kadekoppala,
Kimberly Kline,
Thomas Akompong,
Kasturi Haldar
Publication year - 2000
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.68.4.2328-2332.2000
Subject(s) - plasmodium falciparum , biology , malaria , flow cytometry , parasite hosting , virology , transfection , reporter gene , microbiology and biotechnology , gene , gene expression , immunology , genetics , world wide web , computer science
Stable transfection of a new, chimeric reporter in the human malaria parasitePlasmodium falciparum confers green fluorescence and methotrexate resistance that can be quantitated by Western blotting and flow cytometry. This provides a sensitive, live reporter for exploitation of genomic and high-throughput assays for the identification of new pathogenic determinants.