Open AccessAdherence of Platelets toCandidaSpecies In Vivo
Author(s) -
Raymond Robert,
Sandrine Nail,
A. Marot-Leblond,
Jane Cottin,
M. Miégeville,
S. Quenouillere,
C. Mahaza,
Jean-Marcel Senet
Publication year - 2000
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.68.2.570-576.2000
Subject(s) - candida glabrata , candida albicans , platelet , in vivo , biology , microbiology and biotechnology , candida tropicalis , in vitro , biochemistry , immunology
The in vivo interactions of platelets withCandida species yeast cells were investigated in a murine model. Mice were injected intravenously via the lateral caudal vein, and blood drawn by periorbital puncture was collected in phosphate-buffered saline–formaldehyde to avoid in vitro platelet activation. The study of the clearance of blastoconidia ofCandida albicans andCandida glabrata showed that these cells disappeared quickly from the bloodstream. Microscopic observation of blood samples, stained by Calcofluor white or May Grunwald Giemsa, demonstrated the rapid attachment of platelets to fungal elements of all theCandida spp. tested. The attachment of murine platelets toC. albicans cells, observed by scanning electron microscopy, revealed morphological changes. The platelets lost their discoid shape, generated pseudopodia, and flattened against the yeast cells. The reversibility of platelet binding toC. albicans by chelating agents suggests a cation-dependent link. In contrast, the fixation ofC. glabrata andCandida tropicalis was not modified by chelating agents. The mechanisms involved in the in vivo adherence of platelets toCandida cells may therefore differ according to the species ofCandida .