Open AccessHuman CD8+T Cells Specific forMycobacterium tuberculosisSecreted Antigens in Tuberculosis Patients and Healthy BCG-Vaccinated Controls in The Gambia
Author(s) -
Steven M. Smith,
Michèl R. Klein,
Adam Malin,
Jackson Sillah,
Kris Huygen,
Peter Andersen,
Keith P. W. J. McAdam,
Hazel M. Dockrell
Publication year - 2000
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.68.12.7144-7148.2000
Subject(s) - cytotoxic t cell , mycobacterium tuberculosis , tuberculosis , antigen , biology , immunology , cd8 , perforin , t lymphocyte , bcg vaccine , virology , microbiology and biotechnology , vaccination , medicine , pathology , in vitro , biochemistry
Intracellular flow cytometry analysis of perforin production by CD8+ T cells showed levels were greatly reduced in tuberculosis (TB) patients compared to healthy controls. Reduced cytotoxic-T-lymphocyte activity was also obtained with CD8+ T cells from TB patients compared to healthy controls in The Gambia. A change in antigen recognition was noted between the two groups of donors: in addition to recognition of Ag85A and Ag85B, as seen in healthy donors, a prominent ESAT-6 response was found in TB patients.