Biological Activities of Lipopolysaccharides and Lipid A from Rhodospirillaceae

The lipopolysaccharides and free lipid A from several strains of Rhodospirillaceae were assayed comparatively with those of Enterobacteriaceae in a number of biological tests. Free lipid A's from Rhodopseudomonas gelatinosa and Rhodospirillum tenue exhibited strong serological cross-reactions with each other and with free lipid A from Salmonella. Lipid A's from Rhodopseudomonas viridis and Rhodopseudomonas palustris, although cross-reacting with each other, did not do so with either the lipid A of R. gelatinosa or R. tenue or with that of Salmonella. The presence or absence of the above cross-reactions agreed with corresponding similarities or differences in the chemical structure of the lipid A preparations. The lipopolysaccharide of R. gelatinosa was highly toxic for adrenalectomized mice and pyrogenic for rabbits; however, it exhibited no anti-complementary activity. The activity of the R. tenue lipopolysaccharide was very low in both the lethality and pyrogenicity tests. Its corresponding free lipid A also exhibited low pyrogenic activity; however, its lethal toxicity for adrenalectomized mice was considerably higher than that of the intact parent lipopolysaccharide. Both intact lipopolysaccharide and, unexpectedly, the free lipid A exhibited no anti-complementary activity. The lipopolysaccharides of R. viridis and R. palustris were virtually nontoxic for mice and nonpyrogenic for rabbits. Both lipopolysaccharides were highly potent in their interaction with complement. They therefore represent the first example of nontoxic lipopolysaccharides exhibiting high anti-complementary activity.