Open AccessEscherichia coli Cytotoxic Necrotizing Factor 1 Blocks Cell Cycle G 2 /M Transition in Uroepithelial Cells
Author(s) -
Loredana Falzano,
Perla Filippini,
Sara Travaglione,
Alessandro Giamboi Miraglia,
Alessia Fabbri,
Carla Fiorentini
Publication year - 2006
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.01413-05
Subject(s) - biology , cell cycle , microbiology and biotechnology , cytoplasm , cytotoxic t cell , cell cycle protein , rhoa , cell , escherichia coli , context (archaeology) , actin cytoskeleton , cdc42 , cytoskeleton , actin , signal transduction , biochemistry , in vitro , gene , paleontology
Evidence is accumulating that a growing number of bacterial toxins act by modulating the eukaryotic cell cycle machinery. In this context, we provide evidence that a protein toxin named cytotoxic necrotizing factor 1 (CNF1) from uropathogenicEscherichia coli is able to block cell cycle G2 /M transition in the uroepithelial cell line T24. CNF1 permanently activates the small GTP-binding proteins of the Rho family that, beside controlling the actin cytoskeleton organization, also play a pivotal role in a large number of other cellular processes, including cell cycle regulation. The results reported here show that CNF1 is able to induce the accumulation of cells in the G2 /M phase by sequestering cyclin B1 in the cytoplasm and down-regulating its expression. The possible role played by the Rho GTPases in the toxin-induced cell cycle deregulation has been investigated and discussed. The activity of CNF1 on cell cycle progression can offer a novel view ofE. coli pathogenicity.