
The Salmonella enterica Serotype Typhi Vi Capsular Antigen Is Expressed after the Bacterium Enters the Ileal Mucosa
Author(s) -
Quynh Tran,
Gabriel Gomez,
Sangeeta Khare,
Sara D. Lawhon,
Manuela Raffatellu,
Andreas J. Bäumler,
Dharani K. Ajithdoss,
Soma S. Dhavala,
L. Garry Adams
Publication year - 2010
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.00972-09
Subject(s) - biology , microbiology and biotechnology , salmonella enterica , antigen , serotype , salmonella typhi , intestinal mucosa , salmonella , virulence , enterobacteriaceae , typhoid fever , virulence factor , bacteria , virology , escherichia coli , gene , immunology , medicine , biochemistry , genetics
Salmonella enterica serotype Typhi, the etiological agent of typhoid fever, produces the Vi capsular antigen, a virulence factor absent inSalmonella enterica serotype Typhimurium. Previous studies suggest that the capsule-encodingviaB locus reduces inflammatory responses in intestinal tissue; however, there are currently no data regarding the in vivo expression of this locus. Here we implemented direct and indirect methods to localize and detect Vi antigen expression within polarized intestinal epithelial cells and in the bovine ileal mucosa. We report thattviB , a gene necessary for Vi production inS. Typhi, was significantly upregulated during invasion of intestinal epithelial cells in vitro. During infection of bovine ligated loops,tviB was expressed at levels significantly higher in calf tissue than those in the inoculum. The presence of the Vi capsular antigen was detected in calf ileal tissue via fluorescence microscopy. Together, these results support the concept that expression of the Vi capsular antigen is induced whenS. Typhi transits from the intestinal lumen into the ileal mucosa.