Open AccessDisparate Immunoregulatory Potentials for Double-Negative (CD4−CD8−) αβ and γδ T Cells from Human Patients with Cutaneous Leishmaniasis
Author(s) -
Lis Ribeiro do Valle Antonelli,
Walderez O. Dutra,
Ricardo Riccio Oliveira,
Karen Torres,
Luiz Henrique Guimarães,
Olı́via Bacellar,
Kenneth J. Gollob
Publication year - 2006
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.00890-06
Subject(s) - biology , cd8 , immunology , cutaneous leishmaniasis , t cell , population , cytokine , immune system , cytotoxic t cell , leishmania major , leishmania , antigen , t cell receptor , interleukin 10 , leishmaniasis , medicine , in vitro , parasite hosting , environmental health , world wide web , computer science , biochemistry
Although most T lymphocytes express the αβ T-cell receptor and either CD4 or CD8 molecules, a small population of cells lacking these coreceptors, CD4− CD8− (double negative [DN]) T cells, has been identified in the peripheral immune system of mice and humans. To better understand the role that this population may have in the human immune response againstLeishmania spp., a detailed study defining the activation state, cytokine profile, and the heterogeneity of DN T cells bearing αβ or γδ T-cell receptors was performed with a group of well-defined cutaneous leishmaniasis patients. Strikingly, on average 75% of DN T cells from cutaneous leishmaniasis patients expressed the αβ T-cell receptor, with the remainder expressing the γδ receptor, while healthy donors displayed the opposite distribution with ∼75% of the DN T cells expressing the γδ T-cell receptor. Additionally, αβ DN T cells from cutaneous leishmaniasis patients are compatible with previous antigen exposure and recent activation. Moreover, while αβ DN T cells fromLeishmania -infected individuals present a proinflammatory cytokine profile, γδ DN T cells express a regulatory profile exemplified by interleukin-10 production. The balance between these subpopulations could allow for the formation of an effective cellular response while limiting its pathogenic potential.