Open AccessSwapping of Periplasmic Domains between Brucella suis VirB8 and a pSB102 VirB8 Homologue Allows Heterologous Complementation
Author(s) -
Gilles Patey,
Zhong Qi,
Gisèle Bourg,
Christian Baron,
David O’Callaghan
Publication year - 2006
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.00584-06
Subject(s) - biology , heterologous , brucella suis , periplasmic space , complementation , virology , brucella abortus , microbiology and biotechnology , brucella melitensis , brucella , genetics , gene , brucellosis , mutant , escherichia coli
ABrucella suis mutant with a nonpolar deletion in thevirB8 gene was attenuated in a macrophage infection model. Complementation with theB. suis VirB8 protein expressed from thevirB promoter restored virulence. Expression of TraJ, a VirB8 homologue from plasmid pSB102, did not restore virulence; however, virulence was partially restored by a chimeric protein containing the N terminus of theB. suis VirB8 protein and the C-terminal periplasmic domain of TraJ.