Swapping of Periplasmic Domains between Brucella suis VirB8 and a pSB102 VirB8 Homologue Allows Heterologous Complementation | Zendy

Gilles Patey | Zendy; Zhong Qi | Zendy; Gisèle Bourg | Zendy; Christian Baron | Zendy; David O’Callaghan | Zendy

Open Access

Swapping of Periplasmic Domains between Brucella suis VirB8 and a pSB102 VirB8 Homologue Allows Heterologous Complementation

Author(s) -

Gilles Patey,

Zhong Qi,

Gisèle Bourg,

Christian Baron,

David O’Callaghan

Publication year - 2006

Publication title -

infection and immunity

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.508

H-Index - 220

eISSN - 1070-6313

pISSN - 0019-9567

DOI - 10.1128/iai.00584-06

Subject(s) - biology , heterologous , brucella suis , periplasmic space , complementation , virology , brucella abortus , microbiology and biotechnology , brucella melitensis , brucella , genetics , gene , brucellosis , mutant , escherichia coli

ABrucella suis mutant with a nonpolar deletion in thevirB8 gene was attenuated in a macrophage infection model. Complementation with theB. suis VirB8 protein expressed from thevirB promoter restored virulence. Expression of TraJ, a VirB8 homologue from plasmid pSB102, did not restore virulence; however, virulence was partially restored by a chimeric protein containing the N terminus of theB. suis VirB8 protein and the C-terminal periplasmic domain of TraJ.

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