Open AccessDistinctive Profiles of Infection and Pathology in Hamsters Infected with Clostridium difficile Strains 630 and B1
Author(s) -
David Goulding,
Harold Lincoln Thompson,
Jenny E. Emerson,
Neil F. Fairweather,
Gordon Dougan,
Gill Douce
Publication year - 2009
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.00551-09
Subject(s) - biology , bacteria , microbiology and biotechnology , clostridium difficile , clostridium , hamster , mesocricetus , antibiotics , anaerobic bacteria , pathology , medicine , genetics
Currently, the Golden Syrian hamster is widely considered an important model ofClostridium difficile disease, as oral infection of this animal pretreated with antibiotics reproduces many of the symptoms observed in humans. TwoC. difficile strains, B1 and 630, showed significant differences in the progression and severity of disease in this model. B1-infected hamsters exhibited more severe pathology and a shorter time to death than hamsters infected with 630. Histological changes in the gut did not correlate with absolute numbers ofC. difficile bacteria, but there were clear differences in the distribution of bacteria within gut tissues. Light, scanning, and transmission electron microscopy revealed high numbers of B1 bacteria at the mucosal surface of the tissue, whereas 630 bacteria were more frequently associated with the crypt regions. Both B1 and 630 bacteria were frequently observed within polymorphonuclear leukocytes, although, interestingly, a space frequently separated B1 bacteria from the phagosome wall, a phenomenon not observed with 630. However, pilus-like structures were detected on 630 located in the crypts of the gut tissue. Furthermore, B1 bacteria, but not 630 bacteria, were found within nonphagocytic cells, including enterocytes and muscle cells.