Open AccessProtection Elicited by Two Glutamine Auxotrophs of Mycobacterium tuberculosis and In Vivo Growth Phenotypes of the Four Unique Glutamine Synthetase Mutants in a Murine Model
Author(s) -
Sunhee Lee,
Bo Young Jeon,
Svetoslav Bardarov,
Mei Chen,
Sheldon L. Morris,
William R. Jacobs
Publication year - 2006
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.00531-06
Subject(s) - auxotrophy , biology , glutamine , mycobacterium tuberculosis , glutamine synthetase , mutant , microbiology and biotechnology , mycobacterium bovis , virulence , bcg vaccine , phenotype , tuberculosis , virology , vaccination , biochemistry , amino acid , gene , medicine , pathology
We generated four individual glutamine synthetase (GS) mutants (ΔglnA1 , ΔglnA2 , ΔglnA3 , and ΔglnA4 ) and one triple mutant (ΔglnA1EA2 ) ofMycobacterium tuberculosis to investigate the roles of GS enzymes. Subcutaneous immunization with the ΔglnA1EA2 and ΔglnA1 glutamine auxotrophic mutants conferred protection on C57BL/6 mice against an aerosol challenge with virulentM. tuberculosis , which was comparable to that provided byMycobacterium bovis BCG vaccination.