Open AccessIdentification of a LolC Homologue in Burkholderia pseudomallei , a Novel Protective Antigen for Melioidosis
Author(s) -
David N. Harland,
Karen K. Chu,
Ashraful Haque,
Michelle Nelson,
Nicola Walker,
Mitali SarkarTyson,
Timothy P. Atkins,
Benjamin L. Moore,
Katherine A. Brown,
Gregory J. Bancroft,
Richard W. Titball,
Helen S. Atkins
Publication year - 2007
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.00404-07
Subject(s) - burkholderia pseudomallei , melioidosis , biology , microbiology and biotechnology , antigen , immune system , virology , adjuvant , heterologous , immunology , bacteria , gene , genetics , biochemistry
Melioidosis is an emerging disease of humans in Southeast Asia and tropical Australia. The bacterium causing this disease,Burkholderia pseudomallei , is also considered a bioterrorism agent, and as yet there is no licensed vaccine for preventingB. pseudomallei infection. In this study, we evaluated selected proteins (LolC, PotF, and OppA) of the ATP-binding cassette systems ofB. pseudomallei as candidate vaccine antigens. Nonmembrane regions of theB. pseudomallei proteins were expressed and purified fromEscherichia coli and then evaluated as vaccine candidates in an established mouse model ofB. pseudomallei infection. When delivered with the monophosphoryl lipid A-trehalose dicorynomycolate adjuvant, the proteins stimulated antigen-specific humoral and cellular immune responses. Immunization with LolC or PotF protein domains afforded significant protection against a subsequent challenge withB. pseudomallei . The most promising vaccine candidate, LolC, provided a greater level of protection when it was administered with immune-stimulating complexes complexed with CpG oligodeoxynucleotide 10103. Immunization with LolC also protected against a subsequent challenge with a heterologous strain ofB. pseudomallei , demonstrating the potential utility of this protein as a vaccine antigen for melioidosis.