Open AccesscDNA Microarray Analysis of Host-Pathogen Interactions in a Porcine In Vitro Model forToxoplasma gondiiInfection
Author(s) -
Margaret Okomo-Adhiambo,
Craig W. Beattie,
Anette Rink
Publication year - 2006
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.00386-05
Subject(s) - biology , toxoplasma gondii , complementary dna , microarray analysis techniques , gene , gene expression , microarray , gene expression profiling , pathogen , proinflammatory cytokine , immune system , expressed sequence tag , microbiology and biotechnology , immunology , genetics , antibody , inflammation
Toxoplasma gondii induces the expression of proinflammatory cytokines, reorganizes organelles, scavenges nutrients, and inhibits apoptosis in infected host cells. We used a cDNA microarray of 420 annotated porcine expressed sequence tags to analyze the molecular basis of these changes at eight time points over a 72-hour period in porcine kidney epithelial (PK13) cells infected withT. gondii . A total of 401 genes with Cy3 and Cy5 spot intensities of ≥500 were selected for analysis, of which 263 (65.6%) were induced ≥2-fold (expression ratio, ≥2.0;P ≤ 0.05 [t test]) over at least one time point and 48 (12%) were significantly down-regulated. At least 12 functional categories of genes were modulated (up- or down-regulated) byT. gondii . The majority of induced genes were clustered as transcription, signal transduction, host immune response, nutrient metabolism, and apoptosis related. The expression of selected genes altered byT. gondii was validated by quantitative real-time reverse transcription-PCR. These results suggest that significant changes in gene expression occur in response toT. gondii infection in PK13 cells, facilitating further analysis of host-pathogen interactions in toxoplasmosis in a secondary host.