The akuB KU80 Mutant Deficient for Nonhomologous End Joining Is a Powerful Tool for Analyzing Pathogenicity in Aspergillus fumigatus | Zendy

Márcia Eliana da Silva Ferreira | Zendy; Márcia Regina von Zeska Kress | Zendy; Marcela Savoldi | Zendy; Maria Helena S. Goldman | Zendy; Albert Härtl | Zendy; Thorsten Heinekamp | Zendy; Axel A. Brakhage | Zendy; Gustavo H. Goldman | Zendy

Open Access

The akuB ^KU80 Mutant Deficient for Nonhomologous End Joining Is a Powerful Tool for Analyzing Pathogenicity in Aspergillus fumigatus

Author(s) -

Márcia Eliana da Silva Ferreira,

Márcia Regina von Zeska Kress,

Marcela Savoldi,

Maria Helena S. Goldman,

Albert Härtl,

Thorsten Heinekamp,

Axel A. Brakhage,

Gustavo H. Goldman

Publication year - 2006

Publication title -

eukaryotic cell

Language(s) - English

Resource type - Journals

eISSN - 1535-9778

pISSN - 1535-9786

DOI - 10.1128/ec.5.1.207-211.2006

Subject(s) - ku80 , biology , aspergillus fumigatus , mutant , homologous recombination , non homologous end joining , microbiology and biotechnology , gene , genetics , dna binding protein , transcription factor

To increase the frequency of homologous recombination, we inactivated the KU80 homologue in Aspergillus fumigatus (named akuB(KU80)). Homologous integration reached about 80% for both calcineurin A (calA) and polyketide synthase pksP (alb1) genes in the akuB(KU80) mutant to 3 and 5%, respectively, when using a wild-type A. fumigatus strain. Deletion of akuB(KU80) had no influence on pathogenicity in a low-dose murine infection model.

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