Novel Method for Preparing Spheroplasts from Cells with an Internal Cellulosic Cell Wall | Zendy

Alvin Chun Man Kwok | Zendy; Carmen C. M. Mak | Zendy; Francis T. W. Wong | Zendy; Joseph T.Y. Wong | Zendy

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Novel Method for Preparing Spheroplasts from Cells with an Internal Cellulosic Cell Wall

Author(s) -

Alvin Chun Man Kwok,

Carmen C. M. Mak,

Francis T. W. Wong,

Joseph T.Y. Wong

Publication year - 2007

Publication title -

eukaryotic cell

Language(s) - English

Resource type - Journals

eISSN - 1535-9778

pISSN - 1535-9786

DOI - 10.1128/ec.00301-06

Subject(s) - spheroplast , protoplast , polyethylene glycol , cell wall , cellulose , biology , macromolecule , agar , biophysics , peg ratio , oligonucleotide , yeast , biochemistry , microbiology and biotechnology , fluorescence , gene , bacteria , genetics , physics , finance , escherichia coli , quantum mechanics , economics

Protoplast and spheroplast preparations allow the transfer of macromolecules into cells and provide the basis for the generation of engineered organisms. Crypthecodinium cohnii cells harvested from polyethylene glycol-containing agar plates possessed significantly lower levels of cellulose in their cortical layers, which facilitated the delivery of fluorescence-labeled oligonucleotides into these cells.

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