Open AccessNovel Method for Preparing Spheroplasts from Cells with an Internal Cellulosic Cell Wall
Author(s) -
Alvin Chun Man Kwok,
Carmen C. M. Mak,
Francis T. W. Wong,
Joseph T.Y. Wong
Publication year - 2007
Publication title -
eukaryotic cell
Language(s) - English
Resource type - Journals
eISSN - 1535-9778
pISSN - 1535-9786
DOI - 10.1128/ec.00301-06
Subject(s) - spheroplast , protoplast , polyethylene glycol , cell wall , cellulose , biology , macromolecule , agar , biophysics , peg ratio , oligonucleotide , yeast , biochemistry , microbiology and biotechnology , fluorescence , gene , bacteria , genetics , physics , finance , escherichia coli , quantum mechanics , economics
Protoplast and spheroplast preparations allow the transfer of macromolecules into cells and provide the basis for the generation of engineered organisms. Crypthecodinium cohnii cells harvested from polyethylene glycol-containing agar plates possessed significantly lower levels of cellulose in their cortical layers, which facilitated the delivery of fluorescence-labeled oligonucleotides into these cells.