Open AccessUsing Morpholinos for Gene Knockdown in Giardia intestinalis
Author(s) -
Meredith L. Carpenter,
W. Zacheus Cande
Publication year - 2009
Publication title -
eukaryotic cell
Language(s) - English
Resource type - Journals
eISSN - 1535-9778
pISSN - 1535-9786
DOI - 10.1128/ec.00041-09
Subject(s) - morpholino , biology , electroporation , green fluorescent protein , kinesin , gene knockdown , translation (biology) , reporter gene , epitope , microbiology and biotechnology , gene , gene expression , antibody , biochemistry , messenger rna , genetics , microtubule
We used translation-blocking morpholinos to reduce protein levels inGiardia intestinalis . Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter for morpholino efficacy with targets lacking specific antibodies.