Using Morpholinos for Gene Knockdown in Giardia intestinalis | Zendy

Meredith L. Carpenter | Zendy; W. Zacheus Cande | Zendy

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Using Morpholinos for Gene Knockdown in Giardia intestinalis

Author(s) -

Meredith L. Carpenter,

W. Zacheus Cande

Publication year - 2009

Publication title -

eukaryotic cell

Language(s) - English

Resource type - Journals

eISSN - 1535-9778

pISSN - 1535-9786

DOI - 10.1128/ec.00041-09

Subject(s) - morpholino , biology , electroporation , green fluorescent protein , kinesin , gene knockdown , translation (biology) , reporter gene , epitope , microbiology and biotechnology , gene , gene expression , antibody , biochemistry , messenger rna , genetics , microtubule

We used translation-blocking morpholinos to reduce protein levels inGiardia intestinalis . Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter for morpholino efficacy with targets lacking specific antibodies.

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