Protection against Lethal Leptospirosis after Vaccination with LipL32 Coupled or Coadministered with the B Subunit of Escherichia coli Heat-Labile Enterotoxin | Zendy

André Alex Grassmann | Zendy; Samuel Rodrigues Félix | Zendy; Carolina Ximendes dos Santos | Zendy; Marta Gonçalves Amaral | Zendy; Amilton Clair Pinto Seixas Neto | Zendy; Michel Q. Fagundes | Zendy; Fabiana K. Seixas | Zendy; Éverton F. da Silva | Zendy; Fabrício Rochedo Conceição | Zendy; Odir Antônio Dellagostin | Zendy

Open Access

Protection against Lethal Leptospirosis after Vaccination with LipL32 Coupled or Coadministered with the B Subunit of Escherichia coli Heat-Labile Enterotoxin

Author(s) -

André Alex Grassmann,

Samuel Rodrigues Félix,

Carolina Ximendes dos Santos,

Marta Gonçalves Amaral,

Amilton Clair Pinto Seixas Neto,

Michel Q. Fagundes,

Fabiana K. Seixas,

Éverton F. da Silva,

Fabrício Rochedo Conceição,

Odir Antônio Dellagostin

Publication year - 2012

Publication title -

clinical and vaccine immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.649

H-Index - 77

eISSN - 1556-6811

pISSN - 1556-679X

DOI - 10.1128/cvi.05720-11

Subject(s) - leptospira interrogans , heat labile enterotoxin , leptospira , enterotoxin , leptospirosis , vaccination , microbiology and biotechnology , protein subunit , immune system , escherichia coli , biology , mesocricetus , virology , recombinant dna , antigen , zoonosis , immunology , hamster , gene , biochemistry

Leptospirosis, a worldwide zoonosis, lacks an effective, safe, and cross-protective vaccine. LipL32, the most abundant, immunogenic, and conserved surface lipoprotein present in all pathogenic species of Leptospira, is a promising antigen candidate for a recombinant vaccine. However, several studies have reported a lack of protection when this protein is used as a subunit vaccine. In an attempt to enhance the immune response, we used LipL32 coupled to or coadministered with the B subunit of the Escherichia coli heat-labile enterotoxin (LTB) in a hamster model of leptospirosis. After homologous challenge with 5× the 50% lethal dose (LD(50)) of Leptospira interrogans, animals vaccinated with LipL32 coadministered with LTB and LTB::LipL32 had significantly higher survival rates (P < 0.05) than animals from the control group. This is the first report of a protective immune response afforded by a subunit vaccine using LipL32 and represents an important contribution toward the development of improved leptospirosis vaccines.

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