Next-Generation Sequencing Reveals a Controlled Immune Response to Zaire Ebola Virus Challenge in Cynomolgus Macaques Immunized with Vesicular Stomatitis Virus Expressing Zaire Ebola Virus Glycoprotein (VSVΔG/EBOVgp) | Zendy

Fredrik Barrenäs | Zendy; Richard Green | Zendy; Matthew J. Thomas | Zendy; G. Lynn Law | Zendy; Sean Proll | Zendy; Flora Engelmann | Zendy; Ilhem Messaoudi | Zendy; Andrea Marzi | Zendy; Heinz Feldmann | Zendy; Michael G. Katze | Zendy

Open Access

Next-Generation Sequencing Reveals a Controlled Immune Response to Zaire Ebola Virus Challenge in Cynomolgus Macaques Immunized with Vesicular Stomatitis Virus Expressing Zaire Ebola Virus Glycoprotein (VSVΔG/EBOVgp)

Author(s) -

Fredrik Barrenäs,

Richard Green,

Matthew J. Thomas,

G. Lynn Law,

Sean Proll,

Flora Engelmann,

Ilhem Messaoudi,

Andrea Marzi,

Heinz Feldmann,

Michael G. Katze

Publication year - 2015

Publication title -

clinical and vaccine immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.649

H-Index - 77

eISSN - 1556-6811

pISSN - 1556-679X

DOI - 10.1128/cvi.00733-14

Subject(s) - ebola virus , vesicular stomatitis virus , virology , ebolavirus , immune system , ebola vaccine , biology , virus , mononegavirales , immunization , vesicular stomatitis , vesicular stomatitis indiana virus , immunology , viral disease , paramyxoviridae

Vesicular stomatitis virus expressing Zaire Ebola virus (EBOV) glycoprotein (VSVΔG/EBOVgp) could be used as a vaccine to meet the 2014 Ebola virus outbreak. To characterize the host response to this vaccine, we used mRNA sequencing to analyze peripheral blood mononuclear cells (PBMCs) from cynomolgus macaques after VSVΔG/EBOVgp immunization and subsequent EBOV challenge. We found a controlled transcriptional response that transitioned to immune regulation as the EBOV was cleared. This observation supports the safety of the vaccine.

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